DNA removal & magnetic grain technique
DNA removal is just one of the vital and essential experimental action in the biomedical area. The magnetic grain DNA extraction set makes use of an unique magnetic bead and buffer system. These magnetic beads are particularly covered and have considerable fondness for the target nucleic acid under certain conditions. When problems transform, the magnetic grains will launch the adsorbed nucleic acids, accomplishing the purpose of quick splitting up and filtration of top notch nucleic acids. This reliable magnetic grain modern technology makes it easier and much more reputable to extract pure DNA from examples.
(LingJun DNA Extraction kit)
Magnetic grain approach for DNA extraction steps:
1) Lysis: First, add the ground and broken cells to the lysis service and allow it stand at room temperature for 5-10 mins.
2) Binding: After that add isopropyl alcohol and magnetic grain suspension to the lysis solution and mix by inverting for 5 minutes. Then, position the centrifuge tube on a magnetic stand and wait until the magnetic beads entirely adsorb the target DNA prior to discarding the supernatant.
3) Rinse: Get rid of the centrifuge tube, include rinse service and vortex to resuspend the magnetic grains. After that, put the centrifuge tube on the magnetic stand once more. Dispose of the supernatant after the magnetic beads are entirely taken in. Repeat this step as soon as, making certain that all waste liquid is aspirated, as the residual liquid might influence subsequent experiments. Finally, allow dry for 2 minutes.
4) Elution: Remove the centrifuge tube, include TE barrier, vortex and pipette the magnetic beads to entirely disperse them in the fluid. Then, position it at 56 ° C for 5 mins, shaking and blending every 2 minutes during this period to make certain that the magnetic beads are evenly suspended. Finally, the DNA is soaked up by the magnetic grains into a new centrifuge tube through a magnetic stand.
( DNA/RNA Extraction)
DNA extraction & comparison of several removal methods
1) Knowing and grasping magnetic grain DNA removal technology: As the advantages of the magnetic bead method in genomic DNA removal are progressively coming to be a growing number of popular, it is advised to pay close attention to the advancement of this innovation and find out exactly how to operate and enhance the magnetic bead DNA extraction speculative process.
2) Understand high-throughput and automated equipment: As the organic industry’s need for performance and automation rises, knowledge with and proficiency of relevant high-throughput DNA removal tools and automated systems can boost speculative effectiveness and precision.
3) Safe procedure and experimental efficiency: Considering the danger of direct exposure to harmful reagents using conventional techniques, it is recommended to use the magnetic grain technique for DNA removal to minimize the injury to human wellness throughout experimental procedures and improve experimental efficiency and safety and security.
4) Expansion and application of research study fields: Along with genomic DNA removal, magnetic bead methods are also commonly used in condition discovery, genetic diagnosis, forensic identification and various other fields. It is recommended to explore further the potential applications of the magnetic grain method in various biological areas and contribute even more opportunities to the development of the biological market.
( Lingjun Products)
Distributor Introduction
Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction kit.
We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.
Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding and more. We not only provide products but can also undertake OEM, ODM, and other needs. If you need nucleic acid extraction, please feel free to contact us at sales01@lingjunbio.com.
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